APC/C degron instances
Figure 1. Structure of the ABBA motif degron
Structure of S. cerevisiae APC/C activator protein CDH1 (Cdh1) bound to the ABBA motif of APC/C-CDH1 modulator 1 (Acm1) (PDB:4BH6). Structure shows the shared (green), different (red) and specificity determining (purple) ABBA motif-contacting residues between yeast Cdc20 targets and the human Cdc20.
Gene | Protein | Hit | Type | Start | End | Species | Reference | |
---|---|---|---|---|---|---|---|---|
ACM1 | APC/C-CDH1 modulator 1 | skaaqFMLYEEtaeer | ABBA motif | 61 | 66 | S. cerevisiae | 1,2 | |
BUB1 | Mitotic checkpoint serine/threonine-protein kinase BUB1 | slssaFHVFEDgnken | ABBA motif | 527 | 532 | H. sapiens | 3 | |
BUB1B | Mitotic checkpoint serine/threonine-protein kinase BUB1 beta | gpsvpFSIFDEfllse | ABBA motif | 528 | 533 | H. sapiens | 3,4 | |
BUB1B | Mitotic checkpoint serine/threonine-protein kinase BUB1 beta | qnnsrITVFDEnadea | ABBA motif | 272 | 277 | H. sapiens | 5,6 | |
BUB1B | Mitotic checkpoint serine/threonine-protein kinase BUB1 beta | avlpsFTPYVEetarq | ABBA motif | 340 | 345 | H. sapiens | 5,6 | |
CLB5 | S-phase entry cyclin-5 | vsavqKRQIYNDrtaae | ABBA motif | 99 | 105 | S. cerevisiae | 7 | |
Cyclin A | Cyclin-A2 | skqpaFTIHVDeaeke | ABBA motif | 99 | 104 | H. sapiens | 3 |
References
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(2) "Insights into degron recognition by APC/C coactivators from the structure of an Acm1-Cdh1 complex." He J, Chao WC, Zhang Z, Yang J, Cronin N, Barford D. Mol. Cell. 2013 Jun;50 (5):649-60
(3) "The ABBA Motif Binds APC/C Activators and Is Shared by APC/C Substrates and Regulators." Di Fiore B, Davey NE, Hagting A, Izawa D, Mansfeld J, Gibson TJ, Pines J. Dev. Cell. 2015 Feb;32 (3):358-72
(3) "The ABBA Motif Binds APC/C Activators and Is Shared by APC/C Substrates and Regulators." Di Fiore B, Davey NE, Hagting A, Izawa D, Mansfeld J, Gibson TJ, Pines J. Dev. Cell. 2015 Feb;32 (3):358-72
(4) "The Cdc20-binding Phe box of the spindle checkpoint protein BubR1 maintains the mitotic checkpoint complex during mitosis." Diaz-Martinez LA, Tian W, Li B, Warrington R, Jia L, Brautigam CA, Luo X, Yu H. J. Biol. Chem.. 2015 Jan;290 (4):2431-43
(5) "Molecular basis of APC/C regulation by the spindle assembly checkpoint." Alfieri C, Chang L, Zhang Z, Yang J, Maslen S, Skehel M, Barford D. Nature. 2016 Aug; ():
(5) "Molecular basis of APC/C regulation by the spindle assembly checkpoint." Alfieri C, Chang L, Zhang Z, Yang J, Maslen S, Skehel M, Barford D. Nature. 2016 Aug; ():
(6) "Cryo-EM of Mitotic Checkpoint Complex-Bound APC/C Reveals Reciprocal and Conformational Regulation of Ubiquitin Ligation." Yamaguchi M, VanderLinden R, Weissmann F, Qiao R, Dube P, Brown NG, Haselbach D, Zhang W, Sidhu SS, Peters JM, Stark H, Schulman BA. Mol. Cell. 2016 Aug;63 (4):593-607
(7) "Multiple mechanisms determine the order of APC/C substrate degradation in mitosis." Lu D, Hsiao JY, Davey NE, Van Voorhis VA, Foster SA, Tang C, Morgan DO. J. Cell Biol.. 2014 Oct;207 (1):23-39